comparison between amperometric and chemiluminescence methods in detection and dosimetry of cobalt-60 gamma ray emission

نویسندگان

m. shourian institute of biochemistry and biophysics, university of tehran, p.o. box 13145-1384, tehran, iran

h. ghourchian institute of biochemistry and biophysics, university of tehran, p.o. box 13145-1384, tehran, iran

چکیده

background: hydrogen peroxide (h2o2), produced by gamma ray irradiation to watery solution, was used as an analytical parameter for dosimetry of cobalt-60 (60co) radiation. materials and methods: detection of the produced h2o2 was carried out using two methods: an amperometric biosensor fabricated by immobilization of anthraquinone 2-carboxylic acid modified horseradish peroxidase on glassy carbon electrode, and chemiluminescence technique using luminol as a fluorophore and diperiodatocuprate as a catalyst. results: in the first method, at the applied potential of -550 mv (vs. ag/agcl) the biosensor showed the sensitivity of 74.46 na/gray and detection limit of 0.061 gray (r.s.d=3.67% for n=3) towards h2o2 produced by 60co. in the second method, the sensitivity and detection limit for 60co gamma ray was determined to be 2.34 (luminescence intensity/gray) and 0.069 gray (r.s.d=4.16% for n=3), respectively. conclusion: the obtained results revealed that both proposed techniques, with leaner range from 0.25 to 5 grays are applicable for detection and dosimetry of gamma ray from 60co.

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Comparison between Amperometric and Chemiluminescence methods in detection and dosimetry of cobalt-60 gamma ray emission

Background: Hydrogen peroxide (H2O2), produced by gamma ray irradiation to watery solution, was used as an analytical parameter for dosimetry of cobalt-60 (60Co) radiation. Materials and Methods: Detection of the produced H2O2 was carried out using two methods: an amperometric biosensor fabricated by immobilization of anthraquinone 2-carboxylic acid modified horseradish peroxidase on glassy car...

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عنوان ژورنال:
iranian journal of radiation research

جلد ۱۲، شماره ۴، صفحات ۳۷۷-۳۸۲

کلمات کلیدی
[ ' c o b a l t ' , 6 0 , ' g a m m a r a y ' , ' h y d r o g e n p e r o x i d e ' , ' e l e c t r o c h e m i s t r y ' , ' c h e m i l u m i n e s c e n c e ' ]

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